The single-leg knockout round-of-32 format will see Al Nasr, which won its fifth title in April this year after a penalty shootout win over Sohar, drawn against Seeb, which last won the crown in 1998.
The draw ceremony was held at the Kempinski Hotel Muscat under the auspices of H H Sayyid Harib bin Thuwaini al Said, Assistant Secretary-General of the Council of Ministers for Conferences, in the presence of Sheikh Salim al Wahaibi, the Oman Football Association (OFA) chairman and other top officials and club officials.
In the other mouth-watering clashes, two-time winners Saham will take on last year's runner-up Sohar in a Batinah derby.
Record nine-time champions Fanja meets Al Salam while eight-time winners Dhofar faces Oman Professional League rival, Muscat, in their opening round fixtures.
Another five-time winners, Ahli-Sidab, which scraped into the round-of-32 as the last qualifier on Monday, will take on Al Arouba while another heavyweight, Al Suwaiq, is expected to have a smooth sailing ahead as it was drawn against Bashayer, a first-division side. In another exciting clash featuring two top-flight sides, Al Nahda will meet Majees.
The round-of-32 will be followed by another single-leg round-of-16 to be held on October 7.
The quarterfinals will witness home and away legs to be played on November 6 and December 4 while the double-leg semifinals are scheduled to be held on March 16 and April 10 next year.
The final is expected to be held in April.
Round-of-32 fixtures: Al Nasr vs Seeb, Saham vs Sohar, Al Bashayer vs Suwaiq, Fanja vs Al Salam, Al Nahda vs Majees, Al Hamra vs Al Mussanah, Quriyat vs Samail, Salalah vs Rustaq, Al Mudhaibi vs Mirbat, Jalan vs Bahla, Bidiyah vs Al Shabab, Al Wasta vs Yanqul, Diba vs Sur, Al Arouba vs Ahli-Sidab, Dhofar vs Muscat, Nizwa vs Oman.